英文名Polyethylenimine Linear(PEI) MW40000（rapid lysis）貨號KE1098保存條件室溫

線性PEI轉(zhuǎn)染試劑是一種陽離子聚合物，分子量40000，它能與核酸形成復(fù)合物，并使該復(fù)合物進(jìn)入哺乳動物細(xì)胞。線性PEI轉(zhuǎn)染試劑廣泛適用于常見細(xì)胞系，如 HEK-293、HEK293T、 Hep G2、Hela、CHO-K1、COS-1、COS-7、NIH/3T3 和 Sf9 等。該試劑即使在有血清存在的情況下，它仍然能高效的將核酸導(dǎo)入細(xì)胞。我公司提供的線性PEI轉(zhuǎn)染試劑具有如下優(yōu)點：*的轉(zhuǎn)染效率 , 重組蛋白的高表達(dá)水平,與含血清的培養(yǎng)基相兼容, 低細(xì)胞毒性,易于操作。

本產(chǎn)品為速溶型，溶解迅速，配制方便。

說明書

Transfection Reagent Preparation and Storage Recommendations

Transfection Reagent Preparation (1 mg/mL)

Materials

1.1g PEI MAX 40K (kingmorn Catalog#KE1098)

2.1L Milli-Q® water, water for injection (WFI), or comparable biological-grade water

3.1N Sodium Hydroxide, USP

4.Sterile 25 mL plastic pipette

5.0.1µm, 0.2µm, or 0.22µm PES vacuum sterile-filter unit

6.Sterile HDPE or polypropylene storage vials

Equipment

1.1L glass beaker

2.PTFE-coated stir bar

3.Magnetic stir plate

4.1L glass graduated cylinder

5.pH meter

6.Pipette controller

7.Vacuum pump with tubing

Method

1.In 1L glass beaker, suspend 1g of PEI MAX 40K in 900 mL water.

2.Add PTFE-coated stir bar to 1L glass beaker and set stirring to produce small vortex.

3.Wait for PEI MAX 40K to completely dissolve. This typically takes less than 5 minutes.

4.Use 25 mL plastic pipette to add 1N sodium hydroxide dropwise to 1L glass beaker until pH is 6.90 to 7.10.

5.If pH accidently exceeds 7.10, use 1N hydrochloric acid and a new 25 mL plastic pipette to lower pH back to 6.90 to 7.10.

6.Decant solution from 1L glass beaker to 1L graduated cylinder.

7.Add water to 1L graduated cylinder to adjust final volume to 1L.

8.Sterile-filter solution through vacuum membrane.

9.Aliquot solution as desired.

10.Store aliquots at 4°C

Transfection Solution Storage and Shelf Life

At 4°C in a suitable container, the transfection solution will retain its maximum performance for at least six months. For qualitative work, the solution may be suitable for use for up to a year.